Ridgeway science is pleased to offer a new ELISA for the detection of Equine Chorionic Gonadotropin (eCG) or previously known as Pregnant Mare’s Serum Gonadotropin (PMSG). eCG is typically administered to livestock for hormonal synchronization, to aid in ovulation and synchronised timing of insemination for a flock.

The presence of eCG production by a mare indicates activity of endometrial cups, which form during pregnancy. However these cups can remain active for a period of time after a foetus is lost, so other methods must also be used to ensure no false positives.

This assay can be utilised for equine research of reproductive disorders, as a tool for pregnancy detection/reproductive monitoring utilised by veterinarians or breeders, and for testing and quality control measures in eCG production facilities.

About the ELISA…

The Ridgeway Science eCG ELISA is a 96 well solid phase sandwich assay using a polyclonal anti-eCG coating. Your sample containing eCG may first need to be diluted as the range for this assay is 0-1IU/mL, meaning the assay is highly sensitive. The diluted sample is added and incubated. After an incubation stage, the excess sample is washed off and a HRP conjugate is added. After another incubation stage the unbound conjugate is washed off and chromogenic substrate is added. Addition of stop solution gives a colour change proportional to the quantity of eCG in your diluted sample. Simply multiply by your dilution factor for final results.

Features of eCG ELISA:

  • High sensitivity with a very low dynamic range, enabling detection of low levels of eCG
  • Common sandwich procedure familiar to most assay users
  • 100ul of diluted sample needed, so only a small amount of concentrate
  • 2hrs+ incubation time meaning a quick turnaround for same day results
  • All wash buffers and diluents provided
  • 450nm wavelength
  • Sample types to include serum
  • Quantitative
  • Range 0-1IU/mL or 0-1000mIU/mL
  • For detection of native eCG, not recombinant
  • ELISA comes without standards

The eCG ELISA is £399 is NOW only £250!

Discount due to reduced shelf life of current batch, please enquire for more details.

To order eCG ELISA please contact us on +44 1594 563809, email us at or use our contact form here:

Please contact us if you are in need of standards – we are not currently manufacturing them but we may be able to give you some advice on how to make them/where to source reference eCG.